Now Playing Tracks

Enterococcus faecium is an important multidrug-resistant nosocomial pathogen causing biofilm-mediated infections in patients with medical devices. Insight into E. faecium biofilm pathogenesis is pivotal for the development of new strategies to prevent and treat these infections. In several bacteria, a major autolysin is essential for extracellular DNA (eDNA) release in the biofilm matrix, contributing to biofilm attachment and stability. In this study, we identified and functionally characterized the major autolysin of E. faecium E1162 by a bioinformatic genome screen followed by insertional gene disruption of six putative autolysin genes. Insertional inactivation of locus tag EfmE1162_2692 resulted in resistance to lysis, reduced eDNA release, deficient cell attachment, decreased biofilm, decreased cell wall hydrolysis, and significant chaining compared to that of the wild type. Therefore, locus tag EfmE1162_2692 was considered the major autolysin in E. faecium and renamed atlAEfm. In addition, AtlAEfm was implicated in cell surface exposure of Acm, a virulence factor in E. faecium, and thereby facilitates binding to collagen types I and IV. This is a novel feature of enterococcal autolysins not described previously. Furthermore, we identified (and localized) autolysin-independent DNA release in E. faecium that contributes to cell-cell interactions in the atlAEfm mutant and is important for cell separation. In conclusion, AtlAEfm is the major autolysin in E. faecium and contributes to biofilm stability and Acm localization, making AtlAEfm a promising target for treatment of E. faecium biofilm-mediated infections.

Source: mBio

79 notes

  1. b-fangsta reblogged this from themicrobiologist
  2. anushreelumospatronus reblogged this from themicrobiologist
  3. bklynmed reblogged this from scinerds and added:
    Enterococcus faecium is an important multidrug-resistant nosocomial pathogen causing biofilm-mediated infections in...
  4. lepidopterror reblogged this from fyeahmedlab
  5. itsgoodtoseayou reblogged this from fyeahmedlab
  6. argentarachnids reblogged this from scinerds
  7. artmedandtypography reblogged this from biognosis
  8. biognosis reblogged this from scinerds
  9. sync101 reblogged this from scinerds and added:
    The surgeons nightmare
  10. djbl0nde reblogged this from scinerds
  11. laikas-owner reblogged this from scinerds
  12. 2radical3 reblogged this from scinerds and added:
    enterococcus faecium is an important multidrug-resistant nosocomial pathogen
  13. mangy-mongrel reblogged this from scinerds
  14. prez-doe reblogged this from scinerds
  15. soimlostinlife-dang reblogged this from scinerds
  16. nik0las21 reblogged this from scinerds and added:
    (via TumbleOn)
  17. nursingisinmyblood reblogged this from scinerds
  18. gracefree reblogged this from scinerds
  19. contentment-of-cats reblogged this from scinerds
  20. mental-meanderings reblogged this from scinerds
  21. jonespineapple reblogged this from scinerds
  22. historicalmyths reblogged this from scinerds
  23. shannonontheorange reblogged this from scinerds
To Tumblr, Love Pixel Union